Alcohol Dehydrogenase in Plant Response to Drought

Alcohol Dehydrogenase in Plant Response to Drought 1. Introduction Plant growth and productivity is adversely affected by natures wrath in the form of various abiotic and biotic stress factors (e.g. salinity, low temperature, drought, and flooding heat, oxidative stress and heavy metal toxicity). All these stress factors are a menace for plants and prevent them from reaching their full genetic potential and limit the crop productivity worldwide. Abiotic stress is the principal cause of crop failure, decrease average yields for most major crops by more than 50% (Bray, 2000) and causes losses worth hundreds of million dollars each year. In fact these stresses, threaten the sustainability of agricultural industry (Shilpi, 2005). Environmental degradation and climate change have become severe global problems because of the explosive population increases and industrialization in developing countries. To solve this problem, one of the keys is plant biotechnology based on physiology of crop, plant biochemistry, genomics and transgenic technology. This is becoming more and more important for molecular breeding of crops that can tolerate droughts. For this technology, we need to understand plant responses to drought stress at the molecular level. For agricultural and environmental sustainability, it is important to breed or genetically engineer crops with improved stress tolerance. The identification of key genes and that gene can be used directly for engineering transgenic crops with improved drought tolerance. Although a number of candidate genes have been identified in recent years, only very few have been tested in functional assays for a beneficial effect on drought tolerance. In order to assess gene function directly in plant suffering from abiotic stress caused by the drought, proved to be useful. Analysing the functions of these genes is critical for understanding of the molecular mechanisms governing plant stress response and tolerance, ultimately leading to enhancement of stress tolerance in crops through genetic manipulation. In this study, this will be used for overexpression of genes as well as for induced gene silencing, by using GATEWAY technology. A comprehensive investigation of Adh and Pdc induction and the determination of ethanol production during stress treatments would provide valuable information on how ethanol involved in the response to limited water condition. 2. Literature review 2.1. What is stress? Stress in physical terms is defined as mechanical force per unit area applied to an object. In response to the applied stress, an object undergoes a change in the dimension. Biological term is difficult to define in the plant stress. A biological condition, which may be stress for one plant may be optimum for another plant. The most practical definition of a biological stress is an adverse force or a condition, which inhibits the normal functioning and well being of a biological system such as plants (Jones et al., 1989 ) 2.2. Stress signalling pathways The stress is first perceived by the receptors present on the membrane of the plant cells , the signal is then transduced downstream and this results in the generation of second messengers including calcium, reactive oxygen species (ROS) and inositol phosphates. These second messengers, further modulate the intracellular calcium level. This Ca2+ level is sensed by calcium binding proteins, Ca2+ sensors. These sensory proteins then interact with their respective interacting partners often initiating a phosphorylation cascade and target the major stress responsive genes or the transcription factors controlling these genes. The products of these stress genes ultimately lead to plant adaptation and help the plant to survive the unfavourable conditions. Thus, plant responds to stresses as individual cells and synergistically as a whole organism. Stress induced changes in gene expression in turn may participate in the generation of hormones like ABA, salicylic acid and ethylene. The various stress responsive genes can be broadly categorized as early and late induced genes. Early genes are induced within minutes of stress signal perception and often express transiently. In contrast, most of the other genes, which are activated by stress more slowly, i.e. after hours of stress perception are included in the late induced category. These genes include the major stress responsive genes such as RD (responsive to dehydration)/ KIN (cold induced)/COR (cold responsive), which encodes and modulate the LEA-like proteins (late embryogenesis abundant), antioxidants, membrane stabilizing proteins and synthesis of osmoly tes. 2.3. Drought stress Among all abiotic stresses, drought is one of the most serious problems for sustainable agriculture worldwide. The adverse effect of drought stress is reductions in yield as reported in crops such as rice (Oryza sativa) (Brevedan and Egli, 2003), wheat (Triticum aestivum) (Cabuslay et al., 2002), soybean (Glycine max) (Kirigwi et al., 2004), and chickpea (Cicer aerietum) (Khanna-Chopra and Khanna-Chopra, 2004). The adaptive responses to drought must be coordinated at the molecular, cellular, and whole-plant levels. These conditions induce dehydration of plant cells, which may trigger physiological, biochemical and molecular responses against such stresses (Shinozaki and Yamaguchi, 1996). Water deficit is a complex of responses, which depends upon severity and duration of the stress, plant genotype, developmental stage, and environmental factors providing the stress. Yield losses due to drought are highly variable in nature depending on the stress timing, intensity, and duration. Although, different plant species have variable thresholds for stress tolerance, and some of them can successfully tolerate severe stresses and still complete their life cycles, most cultivated crop plant species are highly sensitive and either die or suffer from productivity loss after they are exposed to long periods of stress. It has been estimated that two-thirds of the yield potential of major crops are routinely lost due to unfavourable growing environments ( Shilpi, 2005 ). Plants have evolved a number of strategies to severe drought. These include escape strategies such as avoidance (flowering, deep rooting, enhanced water uptake efficiency, or reduced water loss) as well as tolerance mechanisms. Reduced shoot growth and increased root development could result in increased water absorption and reduced transpiration, thereby maintaining plant tissue water status. In addition to such avoidance mechanisms, plant responses to water shortages can involve changes in biochemical pathways and expression of genes encoding proteins that contribute to drought adaptation. The proteins could be enzymes involved in the synthesis of osmolytes, antioxidants, or hormones such as ABA and others. Such changes can bring about drought tolerance, whereby plants continue to function at the low water potentials caused by water deficit (Hall, 1993). A central response to water deficit is often increased synthesis of ABA, which in turn induces a range of developmental (avoidanc e) and physiological or biochemical (tolerance) mechanisms. There is an ongoing debate as to whether the exploitation of avoidance or tolerance mechanisms should be the focus of plant breeding programmes. However, it appears likely that the exploitation of tolerance mechanisms may be more promising for the stabilization of crop yield under severe drought conditions (Araus et al, 2002). An assortment of genes with diverse functions are induced or repressed by these drought stresses (Bartels and Sunkar, 2005; Yamaguchi and Shinozaki, 2005). Drought tolerance has been shown to be a highly complex trait, regulated expression of multiple genes that may be induced during drought stress and thus more difficult to control and engineer. Plant engineering strategies for abiotic stress tolerance rely on the expression of genes that are involved in signaling and regulatory pathways (Seki and Shinozaki, 2003) or genes that encode proteins conferring stress tolerance (Wang, 2004) or enzymes present in pathways leading to the synthesis of functional and structural metabolites. Current efforts to improve plant stress tolerance by genetic transformation have resulted in several important achievements; however, the genetically complex mechanisms of abiotic stress tolerance make the task extremely difficult. 2.3.1 Physiological and biochemical responses of drought Physiological and biochemical changes at the cellular level that are associated with drought stress include turgor loss, changes in membrane fluidity and composition, changes in solute concentration, and protein and protein-lipid interactions (Chaves et al,2003) . Other physiological effects of drought on plants are the reduction in vegetative growth, in particular shoot growth. Leaf growth is generally more sensitive than the root growth. Reduced leaf expansion is beneficial to plants under water deficit condition, as less leaf area is exposed resulting in reduced transpiration. Many mature plants, for example cotton subjected to drought respond by accelerating senescence and abscission of the older leaves. This process is also known as leaf area adjustment. Regarding root, the relative root growth may undergo enhancement, which facilitates the capacity of the root system to extract more water from deeper soil layers. Plant tissues can maintain turgor during drought by avoiding dehydration, tolerating dehydration or both (Kramer,1995). These forms of stress resistance are controlled by developmental and morphological traits such as root thickness, the ability of roots to penetrate compacted soil layers, and root depth and mass (Pathan, 2004). By contrast, adaptive traits, such as osmotic adjustment and dehydration tolerance, arise in response to water deficit . Reduction of photosynthetic activity, accumulation of organic acids and osmolytes, and changes in carbohydrate metabolism, are typical physiological and biochemical responses to stress. Synthesis of osmoprotectants, osmolytes or compatible solutes is one of the mechanisms of adaptation to water deficit. These molecules, which act as osmotic balancing agents, are accumulated in plant cells in response to drought stress and are subsequently degraded after stress relief (Tabaeizadeh ,1998). 2.3.2 Molecular responses Studies on the molecular responses to water deficit have identified multiple changes in gene expression. Functions for many of these genà ¨ products have been predicted from the deduced amino acid sequence of the genes. Genes expressed during stress are anticipated to promote cellular tolerance of dehydration through protective functions in the cytoplasm, alteration of cellular water potentia1 to promote water uptake, control of ion accumulation, and further regulation of gene expression. Expression of a gene during stress does not guarantee that a gene product promotes the ability of the plant to survive stress. The expression of some genes may result from injury or damage that occurred during stress. Other genes may be induced, but their expression does not alter stress tolerance. Yet others are required for stress tolerance and the accumulation of these gene products is an adaptive response. Complex regulatory and signaling processes, most of which are not understood, control the expression of genes during water deficit. In addition to induction by stress, the expression of water-deficit-associated genes is controlled with respect to tissue, organ, and developmental stage and may be expressed independently of the stress conditions. The regulation of specific processes will also depend upon the experimental conditions of stress application. Stress conditions that are applied in the laboratory may not accurately represent those that occur in the field. Frequently, laboratory stresses are rapid and severe, whereas stress in the field often develops over an extended period of time ( Radin, 1993). These differences must also be evaluated when studying the adaptive value of certain responses. The function of the gene products and the mechanisms of gene expression are intertwined, and both must be understood to fully comprehend the molecular response to water deficit. 2.4. Function of water-stress inducible genes Genes induced during water-stress conditions are thought to function not only in protecting cells from water deficit by the production of important metabolic proteins but also in the regulation of genes for signal transduction in the water-stress response . Thus, these gene products are classified into two groups. The first group includes proteins that probably function in stress tolerance: water channel proteins involved in the movement of water through membranes, the enzymes required for the biosynthesis of various osmoprotectants (sugars, Pro, and Gly-betaine), proteins that may protect macromolecules and membranes (LEA protein, osmotin, antifreeze protein, chaperon, and mRNA binding proteins), proteases for protein turn over (thiol proteases, Clp protease, and ubiquitin), the detoxification enzymes (glutathione S-transferase, soluble epoxide hydrolase, catalase, superoxide dismutase, and ascorbate peroxidase). Some of the stress-inducible genes that encode proteins, such as a key enzyme for Pro biosynthesis, were over expressed in transgenic plants to produce a stress tolerant phenotype of the plants; this indicates that the gene products really function in stress tolerance ( Shinozaki ,1996 ). The second group contains protein factors involved in further regulation of signal transduction and gene expression that probably function in stress response: Most of the regulatory proteins are involved in signal transduction. Now it becomes more important to elucidate the role of these regulatory proteins for further understanding of plant responses to water deficit. Many transcription factor genes were stress inducible, and various transcriptional regulatory mechanisms may function in regulating drought, cold, or high salinity stress signal transduction pathways. These transcription factors could govern expression of stress-inducible genes either cooperatively or independently, and may constitute gene networks in Arabidopsis ( Pathan.2004 ), 2.5. Model plant for studying the drought tolerant Arabidopsis thaliana is a small weed in the mustard family. It has been a convenient for studies in classical genetics for over forty years ( Redei,1975). This flowering plant also has a genome size and genomic organization that recommend it for certain experiments in molecular genetics and it is coming to be widely used as a model organism in plant molecular genetics, development, physiology, and biochemistry. Arabidopsis thaliana provides an excellent experimental plant system for molecular genetics because of its remarkably small genome size and short life cycle. Arabidopsis thaliana, a genetic model plant, has been extensively used for unravelling the molecular basis of stress tolerance. Arabidopsis also proved to be extremely important for assessing functions for individual stress associated genes due to the availability of knock-out mutants and its amenability for genetic transformation. It has been collected or reported in many different regions and climates, ranging from high elevations in the tropics to the cold climate of northern Scandinavia and including locations in Europe, Asia, Africa, Australia, and North America (Kirchheim,1981). Arabidopsis has the smallest known genome among the higher plants. The reasons for a small genome include little repetitive DNA and, in some cases, simpler gene families. Leutwiler et al. (1984) reported that the haploid genome from Arabidopsis (n = 5 chromosomes) contains only roughly 70,000 kilobase pairs (kb). The contrast of the Arabidopsis genome with that of other plants frequently used in molecular genetic work is striking: tobacco, for example, has a haploid nuclear genome of 1,600,000 kb; the pea haploid genome is 4,500,000 kb; and the wheat haploid genome is 5,900,000 kb . The significance of this small DNA content for molecular genetics is that a genomic library of Arabidopsis chromosomal fragments is easy to make, and simple and economical to screen. It is thus rapid and inexpensive to repeatedly screen Arabidopsis genomic libraries. In addition to its remarkably low content of nuclear DNA, Arabidopsis has a genomic organization that makes it uniquely suited to certain ty pes of molecular cloning experiments. All of the properties of the plant small, short generation time, high seed set, ease of growth, self- or cross-fertilization at willmake Arabidopsis a convenient subject for studies in classical genetics. 2.6. Drought related gene Alcohol dehydrogenase and pyruvate decarboxylase are enzyme whose activity has been observed in numerous higher plants including Arabidopsis, maize, pearl millet, sunflower, wheat, and pea (Gottlieb, 1982). In a number of plants, different ADH genes are expressed in various organs, at specific times during development, or in re-sponse to environmental signals. High levels of ADH activity are found in dry seeds and in anaerobically treated seeds (Freeling, 1973. Banuett-Bourrillon .1979), roots (Freeling .1973), and shoots (App, 1958). During periods of anaerobic stress, the enzyme is presumably required by plants for NADH metabolism, via reduction of acetaldehyde to ethanol. With respect to secondary metabolites, ADH is involved in the inter conversion of volatile compounds such as aldehydes and alcohols (Bicsak et al., 1982; Molina et al., 1986; Longhurst et al., 1990). The ethanolic fermentation pathway branches off the main glycolytic pathway at pyruvate. In the first step, pyruvate is the substrate of pyruvate decarboxylase (PDC), yielding CO2 and acetaldehyde. Subsequently, acetaldehyde is reduced to ethanol with the concomitant oxidation of NADH to NAD+ by alcohol dehydrogenase (ADH). Although PDC and ADH gene induction has been demonstrated, ethanol and acetaldehyde production as a result of stress treatment has only been reported for red pine (Pinus resinosa) and birch (Betula spp.) seedlings exposed to sulfur dioxide, water deficiency, freezing, and ozone(Kimmerer and Kozolowski. 1982). Many plants contain more than one ADH gene (Gottlieb, 1982 ), resulting in the expression of different ADH proteins (i.e. ADH isozymes, often designated ADH 1, ADH2, etc. ). The most extensive study of maize Adh genes, AdhI and Adh2, have been cloned and sequenced. The coding sequences of these genes are 82% homologous, interrupted by nine identically positioned introns that differ in sequence and length. The expression of the Arabidopsis Adh gene (Chang and Meyerowitz, 1986; Dolferus et al., 1990) has many features in common with maize Adhl gene (Walker et al., 1987). The two genes have comparable developmental expression pattens, and both have tissue-specific responses to hypoxic stress. In both maize and Arabidopsis, the gene is expressed in seeds, roots, and pollen grains, whereas green aerial plant parts are devoid of detectable levels of ADH activity. In both species, hypoxic induction of the gene occurs in cells of the root system (reviewed by Freeling and Bennett, 1985; Dolferus and Jacobs, 1991; Okimoto et al., 1980;). ADH is induced anaerobically in Arabidopsis (Dolferus, 1985) as in maize. ADH is also induced in both maize root and Arabidopsis callus by the synthetic auxin 2,4-dichlorophenoxyacetic acid (Dolferus,1985. Feeling, 1973). Several approaches have been undertaken to assess the functional role of Adh in development, stress response, and metabolite synthesis. The expression of the alcohol dehydrogenase (Adh) gene is known to be regulated developmentally and to be induced by environmental stresses (Christie et al., 1991; Bucher et al., 1995). Alcohol dehydrogenase (ADH) plays a key enzymatic function in the response to anaerobic conditions in plants (Sachs, Subbaiah, and Saab 1996). A new and exciting aspect of ethanolic fermentation is the suggested involvement in stress signaling and response to environmental stresses other than low oxygen (Tadege et al., 1999). Furthermore, specific analysis of the ADH gene from rice (Oryza sativa), maize, and Arabidopsis showed ADH to be induced by cold (Christie et al., 1991), wounding (Kato-Noguchi, 2001), dehydration (Dolferus et al., 1994), and the phytohormone abscisic acid (ABA; de Bruxelles et al., 1996), in line with the observation from the micro-array experim ents. In Arabidopsis thaliana, Adh overexpression improved the tolerance of hairy roots to low oxygen conditions and was effective in improving root growth (Dennis et al., 2000; Shiao et al., 2002). However, it had no effect on flooding survival (Ismond et al., 2003). Adh over expression in tomato has been shown to modify the balance between Cà ¢Ã¢â‚¬Å¡Ã¢â‚¬  , Adh overexpression in tomato aldehydes and alcohols in ripe fruits (Speirs et al., 1998). Grapevine plants overexpressing Adh displayed a lower sucrose content, a higher degree of polymerization of proanthocyanidins, and a generally increased content of volatile compounds, mainly in carotenoid- and shikimate-derived volatiles (Catherine et al., 2006).

Development of Anna Fitzgerald Character Essay

Adolescence development relies upon many factors. In order to accurately examine its growth, it is useful to look at some developmental theories. Anna Fitzgerald is thirteen years old; however, she is not like any other teenager with some ordinary problems. Anna was born for a specific purpose she was born to save her sister’s life and to serve as a matched tissue donor. When Anna was born, her umbilical cord was collected and since then she was constantly donating blood, stem cells or bone marrow. That resulted in her undergoing more serious and risky procedures. But when she reaches the age 13, she is being told to donate one of her kidneys. Aware of the fact that she was conceived to be a perfect match and ongoing donor for her sister, she wants to have the chance of living her own life. This is when Anna decides to hire a lawyer and to sue her parents to be â€Å"medically emancipated† from her family. Because she loves her sister unconditionally, Anna struggles with her decision. Developmental theories of Piaget, Ericson, Marcia and Freud are very useful, in order to examine the development of Anna Fitzgerald, the character from â€Å"My Sister’s Keeper†. Nature vs. nurture is the first theory that can be applied to Anna’s life. Nature refers to the human biological inheritance and nurture to the environmental experience (Santrock, MacKenzie-Rivers, Malcomson & Leung, 2011). Since she was born for a specific purpose, her parents had already planned her future. To some point of her life, Anna felt it was normal to be a donor and to be in the hospital three to four days a week. Whenever her sister had an emergency, Anna had to be present. The environment Anna lives in is unusual for a teenager. Anna thinks of herself as a total freak. As it is common for teenagers to complain about her look, she states that God must have had some sort of a moody day on her birthday. She sees a big picture of her household. She knows that the environment which she was born in, did not allow her to be a kid. She had to mature fast and act as an adult. It is clear that Anna is going through identity crisis of moratorium. Moratorium stage according to James Marcia is defined by individual exploring different possibilities, yet not being ready to make a commitment to one. In Anna’s case she had plenty of ideas who she would like to be. When asked by her lawyer, where she sees herself in ten years period, she responds: â€Å"There was a time when, like Kate, I’d wanted to be a ballerina. But since then I’ve gone through a thousand different stages: I wanted to be an astronaut. I wanted to be a paleontologist. I wanted to be a backup singer for Aretha Franklin, a member of the Cabinet, a Yellowstone National Park ranger. Now, based on the day, I sometimes want to be a microsurgeon, a poet, a ghost hunter† (Picoult, 2004, p. 412). What strikes the most in her young, yet mature personality is that in ten years period, she would like to be Kate’s sister. Based on Piaget operational stage theory, Anna is clearly capable of using abstract thought. Abstract thought is an adolescence possibility to think outside of the box and see likely outcomes and consequences. Anna knew exactly that by starting the lawsuit, she has a chance of wining the right to decide for her own. Deep inside her, she still wants to help her sister, but knowing the fact that she cannot make her own decisions, made her to go to the extreme and sue her own parents. She is aware of the fact that her decision may have a huge impact on her sister’s life. Perhaps, she will die; however, she is looking at the long term goal. How is the transplant going to affect her life? Is she going to be able to function normally? What if something goes wrong? All this questions were building up inside of her head and did not want to stop. This process of thoughts indicated her ability to think logically by looking at cons and pros of her situation. Based on Ericson psychosocial developmental theory, Anna is going through identity vs. role confusion stage. She is confused of her role in the family. Often reflecting of who she is, and what is the purpose of her life, besides being a perfect match for her sick sister. Anna once said: â€Å"I used to pretend that I was just passing through this family on my way to my real one† (Picoult, 2004, p. 49). This shows how confused she is in terms of her life. Furthermore, this identity confusion grows into her even more upon receiving a long awaited letter of acceptance, into a two weeks hockey summer camp. She is not allowed to go because of her sister’s condition. There is a big chance of Kate going into some health crisis while Anna is gone. It is a difficult time for a thirteen year old girl who is full of energy and is not being able to be just an ordinary adolescence. One can also relate Freud’s theory of development to Anna. The id is one of the structures of human personality. It operates on principles of pleasure and immediate satisfaction regardless of societal rules or other surrounding context (Santrock, MacKenzie-Rivers, Malcomson & Leung, 2011). Anna’s id arises from her frustration to all medical treatments which are done in order to save her older sister, Kate. Frustration is added by her mother who pushes her to donate the kidney for Kate. Yet, from the start of the novel Anna knows the reality which she refuses to face, as result of her inner id: â€Å"On other hand, I was born for specific purpose†¦ I was born because a scientist managed to hook up my mother’s eggs and my father’s sperm to create a specific combination of precious genetic material†¦ specifically, because I could save my sister, Kateâ€Å"(Picoult, 2004, p. 7-8). This inner id, pushes her to rebel against her parents wishes, and results in Anna seeing a lawyer to help her end the suffering and release her from the heavy responsibility towards her sister. This unconscious part of her personality resurfaced, in her reply to the lawyer, when she says: â€Å"Because, she says simply, it never stops† (Picoult, 2004, p. 22). Some may find this very selfish, with total neglect for her sister’s future well being. It results in confrontation with her mother, who tries to make her realize â€Å"You went to a lawyer and made him think is all about you – and it’s not. It’s about us. All of us –â€Å"(Picoult, 2004, p. 54). Thus, her id rises up and does not care if it destroys other people – parents, brother and her sister. In Anna’s case her id prevails over ego. The ego is supposed to negotiate a compromise between her id, current reality and constraints. Anna feels some guilt, as her ego makes her think over and ask herself about her decision regarding kidney. â€Å"I started thinking about this. Would I have to be in the hospital? Would it hurt? Could people live with just one kidney? What if I wound up with kidney failure when I was, like, seventy? Where would I get my spare?†(Picoult, 2004, p.377). Anna’s superego, is supposed to be her moral guide, conscience to do the right thing. It rises up, specifically, when Anna looks at Kate who is becoming weaker and sicker than before and worries about her future and a possibility of her dying. â€Å"What do you think is the best way to die? I don’t want to talk about this, I said. Why? I’m dying. You’re dying. When I frowned, she said, Well, you are. The she grinned. I just happen to be more gifted at it than y ou are†¦ †¦You know, normal people don’t sit around thinking about dying. Liar. Everyone thinks about dying. Everyone thinks about you dying I said. The room went so still†¦ Then a twitchy smile crossed her face. Well, Kate said, at least now you’re telling the truth† (Picoult, 2004, p. 134-135). From this quote it is clear than Anna has difficulty hearing things from Kate, and that her superego is present and possibly regretting the lawsuit action. Perhaps, this is what prompted Anna, to write in her diary that in case of her death, she wants all of her organs to be donated to Kate. In the end, Anna has a car accident and dies, the lawyer who has won the case and got power of attorney, decides to honor Anna’s last wishes: â€Å"I have power of attorney for Anna, he explains, not her parents. And there is a girl upstairs who needs the kidney† (Picoult, 2004, p. 416). Anna’s life ends up tragically. One can assume that she fulfilled her purpos e in her short life, she saved her sister. Since Anna was born, she was a regular donor to her sister. One can observe it as continuity vs. discontinuity development. â€Å"The first time I gave something to my sister, it was a cord blood, and I was a newborn†¦ The next time she relapsed, I was five and I had lymphocytes drawn from me, three times over, because the doctors never seemed to get enough of them the first time around. When that stopped working, they took bone marrow for a transplant. When Kate got infections, I had to donate granulocytes. When she relapsed again, I had to donate peripheral stem cells† (Picoult, 2004, p. 21). One can explain continuity as a process involving a gradual accumulation of behavior or knowledge. Anna, throughout her short life was exposed to medical procedure, terms and responsibilities from the moment of her birth. She was growing up among those circumstances and she never got a chance to be a kid. She had to mature faster. Even her vocabulary was unusual for a thirteen years old girl. In his mind, her lawyer thought â€Å"This girl’s medical vocabulary would put some of my paid experts to shame† (Picoult, 2004, p. 21). Discontinuity is defined as a passing through life stages in a qualitative way. Since Anna’s character is presented just as she is thirteen years old, one can assume that for her to be able to think abstractly, indeed she was at concrete thinking stage in her earlier age. Anna would go through many different stages, perhaps having her case won; she would still donate her kidney. Anna’s life ends abruptly in a car accident. The logical sequence of life is death but to Anna it was way too early. In conclusion, Piaget, Ericson, Marcia and Freud theories were helpful to examine Anna development by using the appropriate key issues. Based on their theories, it is clear to observe Anna’s life and struggles that she is going through. The young age was not an obstacle to deal with some serious adult problems to which Anna was exposed to from an early age. Throughout the story she has dilemmas concerning her sister’s life. By combining the work of these theorists, it was possible to analyze her life from psychological perspective. References Keenan, T. (2011). Developmental psychology lecture. Intro To Developmental Psychology. Niagara Collage. Welland, Ontario, Canada Keenan, T. (2011). Developmental psychology lecture. Theories of Development. Niagara Collage. Welland, Ontario, Canada Keenan, T. (2011). Developmental psychology lecture. Adolescence. Niagara Collage. Welland, Ontario, Canada Picoult, J. (2004). My sister’s keeper. New York, NY: Atria Books. Santrock, J. W., MacKenzie-Rivers, A., Malcomson, T., & Leung, K. H. (2011). Life-span development. (4th ed.). McGraw-Hill Ryerson Ltd.

Friends Essay Samples Help!

Friends Essay Samples Help! Life, Death and Friends Essay Samples Friendships are critical to your mental and physical well-being, and let's face itthey make life a great deal simpler to handle. Sometimes, you can need more than just idle chit chat and that's when friendship gets even more valuable. Excellent friends are always by your side and support you once you are in the perfect track. Laughter is generally a large part of the majority of friendships and if you're especially close then it's normally the case that you have the ability to make each other laugh and will know precisely how to cheer one another up. Unique things to various folks, since the situation demanded. When two folks are jealous of each other they become competitive with one another. It's said prosperity makes friends. It is critical to choose friends wisely as friendship is an important portion of people's life. The Advantages of Friends Essay Samples EEAnyone who has spent time with friends will see that each one has a distinctive personality all their very own. You might just have good friends you may count on sometimes, but to be dependable on an individual and knowing they'll be there for you, it's difficult to find someone like that. Another kind of friend an individual can have, and the ideal kind, is a legitimate friend. The reason there are unique forms of friends is because each individual has a different idea about what a friend is and what they do for one another. The previous kind of friend is the ideal friend. A friend corrects you once you are in the incorrect. He does not necessarily have to be a person. It's difficult to find a friend like that. Friendship is the connection between two or many buddies. It is essential for the successful wellbeing of every person. It is being able to cry together. Friendship is a give and take relationship for sometimes we have to be a buddy to be in a position to gain one. Writing an essay about friendships does not need to be difficult. Stephen's essay is rather effective. Writing an essay is an essential role in academe life. Take into consideration the critical points you want to construct your persuasive essay about friendship on. It is crucial to have somebody with whom you may share your thoughts freely. Put simply, your very best friends make you truly feel good about yourself. The author starts with a rather in depth story of an event or description of an individual or place. Usu ally, an effective response is going to be 150 to 225 words. Occasionally it's helpful to observe how others were able to get over the difficult first-line hump. If you are giving the job of writing anessay about friend, about friends or friendships, you've accepted a great undertaking. Being aware of what to say and when to say is the reason same-sex friendships are far more prevalent than mixed-sex friendships. Finding peers who think in a similar way and revel in the exact same hobbies is the simplest way of beginning a friendship. There are lots of advantages of friendship. Let's take a better look at a number of the things that may demonstrate the genuine value of friendship. A friend also attempts to make you truly feel good about yourself especially whenever you have confidence problems and your ego is shattered. Finally, among the reasons why you always need to value friendship is that a fantastic friend will always be present to lend you a helping hand whenever you require it. There are different sorts of friends. It is where friends are created and hold a significant part in the students' lives. Finally, it's critical to stay oneself whilst making friends. An old friend is similar to old wine. Life, Death and Friends Essay Samples There are three sorts of friends. There are various varieties of friends that one wants or requirements. These kinds of friends aren't very trustworthy nor should they be trusted. Every friend has a different sort of character. What to Expect From Friends Essay Sa mples? Include anything you would like to. You are most likely thinking of a clever way on the best way to escape from the circumstance where you most likely have not ever imagined. When you're surrounded by them, you find that nothing on earth can ever replace them. A friend will enable you to win sometimes especially if he's too fantastic that you beat in a for instance a game like chess. There's no ideal solution on how best to compose an effective essay. The first thing which you've got to do is think of a topic that you wish to right about. In a nutshell, the service exists, so should you need to use it in order to find a top essay, that's reason enough. It's difficult to find a true friend, it requires a due procedure and time to have one. Process will be the easy and believe me you're likely to do it easily. The procedure will be easy and you merely have to adhere to the steps.

The Moral Limits of Market by Michael Sandel - 1063 Words

What Money Can’t Buy; The Moral Limits of Market by Michael Sandel argues the relationship between markets and our morality. His central concern is the influence of money on the sphere of life traditionally governed by nonmarket norms such as rights as a citizen, care for others, and civic duties. He demonstrated that market is responsible for destroying our sense of morality by placing monetary value to it. This paper will argue the relationship between market and morality through demonstrating the type of goods corrupted by money, the flaws in the market system that causes such problems, and the political solution for this problem as suggested by Michael Sandel respectively. Sandel argues, â€Å"almost everything can be bought and sold.†Ã¢â‚¬ ¦show more content†¦Sandel account these examples as economic inequality where â€Å"in a society where everything is for sale, life is harder for modest means.† The market is not equal because society do not have equal amount of wealth. Economists would dismiss such idea, saying the free market revolve around demand and supply, and willingness to buy or sell goods and services. However, Sandel thinks those who willing to pay extra for goods and services always won. Based on above example, those who have the money will certainly be better off whereas those who cannot afford have no choice, but to be content. Thus, this creates an unequal distribution of wealth in the society. Sandel further argues, â€Å"Commercialism erodes commonality.† He points to the division of society through acquisition of certain goods. For example, the skyboxes at baseball stadiums are affordable only to the wealthy, which separate themselves with the rest of the society. He argues that because the wealthy can buy social advantages such as better education, healthcare, and clothing, they create an exclusive grouping that further divide the society. In this case, money changes the perception of goods. Given the more expensive goods are considered of better quality, increasing the price of a certain good will increase its value. This is adamant in our current time where the same product, such as ice cream, is more expensive in the wealthier neighborhood compared to poorer neighborhood. Thus, heShow MoreRelatedJustice Is The Quality Of Being Just967 Words   |  4 Pagesinfluences and have a realistic discussion about what sort of society we really want to live in. My beli efs and ideas about what Justice means addressed in this reflection relate to what I gathered from Justice: What’s the Right Thing to Do by Michael J. Sandel, A Conflict of Visions by Thomas Sowell and Who Rules America? The Triumph of the Corporate Rich by William Domhoff. Figure 1. This picture above speaks volumes. It shows how people rely on justice to be just when it really it does not careRead MoreJohn Rawls and Equality1052 Words   |  5 Pagesdisadvantages. 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